This study describes asymmetry in the main running mechanical variables during repeated treadmill sprints in elite female athletes and examines whether inter-limb differences in sprinting mechanics increase with fatigue. Eighteen elite female players (French national Rugby Sevens team) performed 8 × 5-s sprints (25-s rest) on an instrumented motorised sprint treadmill. The group mean ‘symmetry angle’ (SA) scores were ~1–2% for contact time (1.6 ± 0.6%), aerial time (2.1 ± 0.8%), step frequency (1.3 ± 0.5%) and step length (1.6 ± 0.6%). Mean vertical and horizontal forces, vertical and leg stiffness presented SA values of 1.7 ± 1.5%, 2.4 ± 1.2%, 2.6 ± 0.2% and 2.5 ± 0.2%, respectively. The SA scores were ~2–8% for duration of braking (6.9 ± 5.0%) and propulsive (6.5 ± 4.4%) phases, peak braking (6.5 ± 2.5%) and propulsive (1.6 ± 0.9%) forces as well as net (5.8 ± 5.6%), braking (7.7 ± 5.3%) and propulsive (2.7 ± 1.6%) impulses. However, there was no influence of sprint repetition number on SA scores for tested variables (P > 0.05). In elite female Rugby Sevens players, there was no noticeable difference in asymmetries for the great majority of stride mechanical variables during repeated treadmill sprints.