%0 Journal Article %T Concomitant aerobic- and hypertrophy-related skeletal muscle cell signaling following blood flow-restricted walking %+ University of Guilan %+ University of California (UC) %+ French Institute of Sport (INSEP), Laboratory Sport, Expertise and Performance (EA7370) (SEP (EA7370)) %A Barjaste, A. %A Mirzaei, B. %A Rahmani-Nia, F. %A Haghniyaz, R. %A Brocherie, Franck %< avec comité de lecture %@ 0765-1597 %J Science & Sports %I Elsevier %V 36 %N 2 %P e51-e58 %8 2021-04 %D 2021 %R 10.1016/j.scispo.2020.03.006 %K KAATSU %K Vascular occlusion %K Mitochondrial biogenesis %K Skeletal muscle hypertrophy %K Muscle biopsy %K Submaximal exercise %Z Life Sciences [q-bio] %Z Humanities and Social Sciences/Sport/Sport physiologyJournal articles %X Objective
This study is aimed to examine the acute responses to a blood flow restriction (BFR) low-intensity aerobic exercise, compared with a similar exercise without BFR, on several aerobic- and hypertrophy-related proteins expression and hormonal responses.

Methods
On two different occasions, five healthy untrained male subjects were required to perform (i) a BFR low-intensity aerobic exercise consisting of 5 sets of 2-min bouts of walking at an exercise intensity of 63–65% HRmax (equivalent to 40% of VO2max), interspersed by 1-min rest; and (ii) similar exercise bouts without BFR (Ctrl). For each condition, venous blood samples were collected at pre- (baseline), immediately and 2-h post-exercise. Baseline and 3-h post-exercise muscle biopsy samples (vastus lateralis) were also performed for protein expression analysis.

Results
HIF-1α, PGC-1α, and VEGF proteins content were significantly higher (P < 0.05) at 3-h post-exercise in BFR compared with baseline values. In reference to baseline, phosphorylation of Akt also increased significantly (P < 0.05) following BFR, and in a greater extent (P < 0.05) than following Ctrl. IGF-1 concentration significantly increased (P = 0.001) immediately following BFR exercise than baseline values and serum GH showed a significant increase (P = 0.046) compared with Ctrl.

Conclusion
The addition of blood flow restriction during walking exercise initiate a concomitant cell signaling pathways regulating mitochondrial biogenesis, angiogenesis, and skeletal muscle protein expression. %G English %L hal-04067262 %U https://insep.hal.science//hal-04067262 %~ SHS %~ INSEP